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Nature Communications 2022 Nov 8;13(1):6737. 

Tb ADAT2/ADAT3 deaminase in complex with tRNA 

 

* ADAT :  Adenosine Deaminase Acting on tRNA

 

Nature Communications 2022 Nov 8;13(1):6737.

Structural basis for sequence-independent substrate selection by eukaryotic wobble base tRNA deaminase ADAT2/3.

Luciano G Dolce  ¹ , Aubree A Zimmer  ² , Laura Tengo  ¹ , Félix Weis  ³ ,  

Mary Anne T Rubio  ² , Juan D Alfonzo  ² , Eva Kowalinski  ⁴

Affiliations

• ¹ EMBL Grenoble, 71 Avenue des Martyrs, 38042, Grenoble, France.
• ² Department of Microbiology and The Center for RNA Biology, The Ohio State University, Columbus, OH, USA.
• ³ EMBL Heidelberg, Structural and Computational Biology Unit, Meyerhofstraße 1, 69117, Heidelberg, Germany.
• ⁴ EMBL Grenoble, 71 Avenue des Martyrs, 38042, Grenoble, France. kowalinski@embl.fr.

  PMID: 36347890
PMCID: PMC9643335  (Free PMC Article)
   DOI: 10.1038/s41467-022-34441-z 

 

 

The Cryo-EM structure 8AW3 [3D viewer LINK]

 

 

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Nature 542, 494-497 (23 February 2017)

Editing and methylation at a single site by functionally interdependent activities

Mary Anne T. Rubio, Kirk W. Gaston, Katherine M. McKenney, Ian M.C. Fleming, Zdenek Paris, Patrick A. Limbach and Juan D. Alfonzo

SHARED LINK:  HERE

 

 

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Nature 542, 420-421 (23 February 2017)

Molecular biology: RNA editing packs a one-two punch 

William T. Yewdell & Jayanta Chaudhuri

NEWS AND VIEWS LINK: HERE

 

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 3 October2013

Molecular Cell  

Unusual Noncanonical Intron Editing Is Important for tRNA Splicing in Trypanosoma brucei

How a ‘Mistake’ in a Single-Celled Organism is Actually a Rewrite Essential to Life 

Acknowledgments: Juan Alfonzo, Mary Anne Rubio, Chris Trotta & Craig Foster

    

 

 

"The scientist does not study nature because it is useful; 

he studies it because he delights in it, and he delights in it because it is beautiful. 

If nature were not beautiful, it would not be worth knowing, 

and if nature were not worth knowing, life would not be worth living." 

-Jules Henri Poincare (1854-1912); French mathematician.

OUR INTERESTS:   RNA, RNA and RNA!

WHO: trypanosomes, yeast, mammals, archaebacteria

WHAT: tRNA modification, tRNA editing, tRNA   transport, tRNA maturation

WHEN: preferably all the time

HOW:  biochemistry, microbiology, genetics, bioinformatics, structural biology

WHERE:  Dept. of Microbiology & The Center for RNA Biology @ OSU

tRNA Editing and Modification...location, location, location!

 

Two major mechanisms import tRNAs to mitochondria: (Alfonzo & Soll 2009)

Sweet Summer of 2016

ALFONZOLAB:  Cuatro de Mayo 2013

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